Differentiating between fresh and frozen-thawed fish fillets by mitochondrial permeability measurement

In this study, we investigated the properties of mitochondria to discriminate fresh from frozen-thawed fish fillets. Mitochondria were isolated gilthead seabream fillets and impact freezing was evaluated by measuring permeability mitochondrial membranes. Freezing led permeabilization inner membranes reduced nicotinamide adenine dinucleotide (NADH). The increase in related shock compared physiological stored at 4 °C. Two approaches chosen measure permeability: a spectrophotometric method consumption NADH complex I, an oxygraphic O2 respiratory chains after exposure NADH. highly permeable no longer sensitive membrane permeabilizing agent: alamethicin. Altogether, our scientific approach allowed us that have been exposed or not (−80 °C) thus between